Journal: Cell reports

Article Title: Multi-modal characterization and simulation of human epileptic circuitry

doi: 10.1016/j.celrep.2022.111873

Figure Lengend Snippet:

Article Snippet: Single-neuron biophysical all-active models , Allen Institute for Brain Science , https://portal.brain-map.org/explore/models/perisomatic-single-neurons.

Techniques: Software, Extraction

Journal: bioRxiv

Article Title: DLC1 is a direct target of activated YAP/TAZ that drives collective migration and sprouting angiogenesis

doi: 10.1101/755389

Figure Lengend Snippet: (a) Representative Western blot analysis of DLC1 and β-actin (loading control) in total lysates of HUVECs transduced with different shDLC1 clones (#1067, #1066, #1065, #1064, #1063). (b) Widefield immunofluorescence (IF) images of HUVECs transduced with shControl or shDLC1 (pool of #1063/1064) and stained for VE-cadherin (green) and F-actin (red). (c) Line graph shows the average ± stdev transendothelial impedance measured at 4000 Hz across barrier forming endothelial cells transduced with shControl or shDLC1 (pool of #1063/1064) plated on fibronectin-coated 8W10E ECIS arrays. Representative data is from 2 independent experiments and an average of 6 wells per condition. (d) Boxplot showing quantification of RhoA activity in G-LISA assays in lysates of shControl and shDLC1 transduced HUVECs, with or without thrombin stimulation. Data is from 4 independent experiments. Whiskers show min/max values. n.s.; not significant (Two-tailed unpaired Student’s t-test). (e) Representative widefield IF images of HUVECs transduced with GFP-DLC1 stained for Paxillin pY118 (red) and F-actin (blue). (f) Widefield IF images of shControl and shDLC1 transduced HUVECs and stained for Paxillin pY118 (green), F-actin (blue), VE-cadherin (red). Boxplot showing quantification of manually counted number of focal adhesions per µm2 of shControl and shDLC1 transduced HUVECs. Whiskers show min/max values. Data is from 2 independent experiments, shControl (42 cells from 12 images) and shDLC1 (46 cells from 19 images). **P<0.01 (Two-tailed unpaired Student’s t-test). (g) Left images are stills from time lapse TIRF microscopy at t=0 of HUVECs transduced with shControl and shDLC1 and paxillin-mCherry. Heat map shows the corresponding focal adhesion dynamics over 30 minutes in a unique color per time frame. Note the stability of focal adhesions in shDLC1 HUVECs as indicated by the white colored FAs in the right panels. See corresponding Supplemental Movie 1 for the ∼2.5 hours time-lapse recording. (h) Bar graph showing quantification of focal adhesion lifetime, assembly and disassembly rates based on TIRF time lapse experiments with paxillin-mCherry expressing HUVECs. Error bars are s.e.m. Data is from 2 independent experiments, shControl (6 movies) and shDLC1 (8 movies). Focal adhesion tracking was performed using the focal adhesion analysis webserver . **P<0.01 (Two-tailed unpaired Student’s t-test). (i) DIC images and cell-substrate traction force maps of HUVECs transduced with shControl or shDLC1. Boxplot showing the mean (± upper and lower quartiles) of measured RMS traction forces of shControl (12 image fields) and shDLC1 (18 image fields) endothelial cells. Data is from 3 independent experiments. Whiskers show min/max values. **P<0.01 (Two-tailed unpaired Student’s t-test).

Article Snippet: Cells were seeded on the arrays and the impedance was measured during monolayer formation at 4 kHz using the ECIS model ZTheta (Applied BioPhysics).

Techniques: Western Blot, Control, Transduction, Clone Assay, Immunofluorescence, Staining, Activity Assay, Two Tailed Test, Microscopy, Expressing